2007-12-02T11:38:00.001-08:00

Azerbaijani Blog For Stem Cells
The stem cell niche is a unique tissue microenvironment that regulates the selfrenewal and differentiation of stem cells

It is well known that Oct4 and Sox2 play an important role in the maintenance of embryonic stem cell pluripotency. These transcription factors bind to regulatory regions within hundreds of target genes to control their expression

Zfp206 regulates ES cell gene expression and differentiation

Zfp206 regulates ES cell gene expression and differentiation

Courtesy of engineering.vcu.edu

Azerbaijan

Goy-Gol Landscape, Azerbaijan. Courtesy of hardasan.com

Azerbaijan, Photos

Azerbaijan: News, APA

Azerbaijan: News, ANS

The oldest spots of civilization

Azerbaijan & Tourism

Azerbaijani language

NASIB NASSIBLI (NASIBZADE), Fulbright Fellow, University of Chicago

Shusha History

Khojali's Rosa Is Beautiful

Courtesy of hazenworld.com

Courtesy of angelfire.com

Links:

Occupation of Khojaly city of Azerbaijan by Armenian armed forces turns 17 years

Victims of Khojali Genocide commemorated in foreign countries

The Khojali genocide with its inconceivable cruelty and inhuman punitive methods

On Feb. 24. Armenian troops committed genocide in Khojali city

No more using of dangerous viruses for delivering of the four "Decider's Factors" for induction of reprogramming

Imagine! Imagine about all kinds of viruses. Imagine they are called the "X-things". Viruses are positioned between the nonliving and the living things. Imagine that these viruses, namely X-things have the capability of very functional delivery systems. These "Bezuaberten" (German word for magical) delivery systems send some factors into the genome of a cell. These so -called the "bezauberten factors", definietely, are not going meaningless into the genome of a cell [ to play footbal or ... in the genome!]; They are definietly going to change and modify human's genome, in an egoistic and destructive way; these so-called the "bezauberten factors" of this sort of delivery system show the tendency for destructing of genes. They have a mission; a mission in the genome of a cell. As you know, the result would be very catastrophic. The result will be something like so-called the tumourigenesis.

Stem Cell Breakthrough: Toronto reprograms by jumping genes in and out

Nonviral plasmid-based vectors are free of many of the disadvantages that constrain viral vectors

Gene Delivery to Mammalian Cells: Volume 1: Nonviral Gene Transfer ... - Google Books Result

The magic of four: induction of pluripotent stem cells from somatic cells by Oct4, Sox2, Myc and Klf4

The magic of four: induction of pluripotent stem cells from somatic cells by Oct4, Sox2, Myc and Klf4

The magic continues for the iPS strategy

Regulation of Pluripotency and Reprogramming by Transcription Factors

Enhanced efficiency of generating induced pluripotent stem (iPS ...

Essay Novel Approaches to Epigenetic Reprogramming of Somatic Cells

Induced pluripotent stem cell (iPS) technology: promises and challenges

Sox2 and Oct-3/4 function as master regulators during mammalian embryogenesis

IPS Cells Are Man-Made Cancer Cells

Gene by gene, scientists dig for the triggers

Protein phosphorylation is central to the understanding of cellular signaling, and cellular signaling is suggested to play a major role in the regulation of human embryonic stem (ES) cell pluripotency

Stem cell therapy for heart failure: the science and current progress

Stem Cells and Development iPS Cells: A More Critical Review

The iPS Spinning in Top Journals

The KLF4 tumour suppressor is a transcriptional repressor of p53 that acts as a context-dependent oncogene

Common sense for our genomes

Holy Grail of stem cell research within reach

The low efficiency with which iPS cells are derived hinders studies on the molecular mechanism of reprogramming, and integration of viral transgenes

Epigenetic background of neuronal fate determination

Reprogramming adult cells into pluripotent stem cells - what do these new results mean

Roles of Krüppel-like factor 4 in normal homeostasis, cancer and stem cells

Set of four factors (OCT4, SOX2, NANOG

Adult Skin Cells Reprogrammed into Stem Cells for Disease Research

Artificial Reprogramming of Human Somatic Cells to Generate Pluripotent Stem Cells

High Throughput Microarray Platform

Skin Cells Reprogrammed Into Pluripotent Stem Cells

Signal Transduction Molecules as Targets for Cancer Prevention

Starvation induces healthy cells to go into “protective” mode

A transposon, or jumping gene, is a sequence of DNA that can move to different positions within the genome of a single cell. Much of gene therapy research has focused on different viral modes of gene delivery. While viruses seem well suited to deliver therapeutic genetic material, they are also effective at entering a host organism’s DNA. A significant drawback in using this therapy has been that it can trigger immune reactions or activate cancer-causing gene

The theoretical advantage of using a piggyBac, in contrast, is that the gene can be delivered safely to a specific location in the genome, where it can achieve the desired therapeutic response. Scientists believe that this type of gene therapy could have multiple applications, including the correction of single gene disorders such as muscular dystrophy and hemophilia.

The study, “piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells,” was published in the October 10, 2006 issue of the Proceedings of the National Academy of Sciences

Source:

Gene Therapy Research Focuses on Nonviral Delivery

The piggyBac transposon was five to 10 times better than the other circular pieces of DNA at making a home and a difference in several mammalian cell lines

Helper-Independent Sleeping Beauty Transposon–Transposase Vectors ...

Jumping Gene Could Provide Non-viral Alternative For Gene Therapy

Biochemie: Eine Einführung mit 40 Lerneinheiten - Google Books Result

Genetic and biochemical characteristics of the most commonly used antibiotic resistance
marker genes

The development of gene technology

Ist die Gentherapie als Prinzip zur Behandlung von Erbkrankheiten am Ende?

Self-Regulating Gene Switches and Molecular Evolution

PiggyBAC Express :Jumping Gene Offers Nonviral Alternative to Gene Therapy

Courtesy of medicineworld.org

Dr. Joseph M. Kaminski, an MCG radiation oncologist:“If we want to add a therapeutic gene, we can put it in the transposon and use it to deliver the gene into the cell.You can use these wherever retroviruses have been used.”
The piggyBac transposon, which has close relatives in the human genome, is widely used to genetically modify insects. Sleeping Beauty has been used to correct hereditary diseases, including hemophilia, in a mouse model.

A jumping gene first identified in a cabbageeating moth may provide a safer, targetspecific alternative to viruses for gene therapy, researchers say.In addition to piggyBac, researchers looked at what was believed to be the most efficient transposon in mammalian cells, hyperactive Sleeping Beauty, first found “asleep” in fish. They also looked at Tol2, another fish transposon, and Mos1, found in insects. The piggyBac transposon, which has close relatives in the human genome, is widely used to genetically modify insects. Sleeping Beauty has been used to correct hereditary diseases, including hemophilia, in a mouse model.
For this study, researchers used transposons to deliver an antibiotic resistant gene. They found that while piggyBac might be less efficient than a virus, it puts Sleeping Beauty to shame when it comes to making cells antibioticresistant.

Source:

Jumping Gene Offers Nonviral Alternative to Gene Therapy

Courtesy of medicineworld.org

Transposons: Non-Viral Alternative for Gene Therapy

"If we want to add a therapeutic gene, we can put it within the transposon and use it to deliver the gene into the cell," says Dr. Joseph M. Kaminski

Efficient Transposition of the piggyBac (PB) Transposon in Mammalian Cells and Mice

The piggyBac transposon was five to 10 times better than the other circular pieces of DNA

The piggyBac transposon, which has close relatives in the human genome, is widely used to genetically modify insects

Courtesy of medicineworld.org

Ethical stem cells stripped of 'cancer' genes

It is now possible to create "reprogrammed" stem cells that have been stripped of potentially cancer-causing genes

The technology of cell culture consists of growing of cells with appropriate nutrients

Efficient in vivo expression of a reporter gene in rat brain after injection of recombinant replication-deficient Semliki Forest virus

Die Experten aus Münster haben einen möglichen Meilenstein auf dem Weg zu einer Stammzelltherapie erreicht

Forscher haben eine Methode gefunden, Stammzellen herzustellen – ohne Krebsgefahr. Doch es sind immer noch nicht alle Probleme gelöst

Forscher programmieren Stammzellen um

Scientists have coaxed human skin cells into mimicking the disease-fighting potential of embryonic stem cells

Genetic engineering stock photos and images

Medical Research News Blog From Medicineworld.Org

Stem Cell Breakthrough: New Method For Creating Stem Cells,ScienceDaily (Mar. 1, 2009)

Dr. A. Nagy. Courtesy of CTV.ca

Toronto, Mount Sinai Hospital's Dr. Andras Nagy ScienceDaily (Mar. 1, 2009): This new method of generating stem cells does not equire embryos as starting points and could be used to generate cells from many adult tissues such as a patient's own skin cells."

ScienceDaily (Mar. 1, 2009) — Mount Sinai Hospital's Dr. Andras Nagy discovered a new method of creating stem cells that could lead to possible cures for devastating diseases including spinal cord injury, macular degeneration, diabetes and Parkinson's disease. The study, published by Nature, accelerates stem cell technology and provides a road map for new clinical approaches to regenerative medicine.

"We hope that these stem cells will form the basis for treatment for many diseases and conditions that are currently considered incurable," said Dr. Nagy, Senior Investigator at the Samuel Lunenfeld Research Institute of Mount Sinai Hospital, Investigator at the McEwen Centre for Regenerative Medicine, and Canada Research Chair in Stem Cells and Regeneration. "This new method of generating stem cells does not require embryos as starting points and could be used to generate cells from many adult tissues such as a patient's own skin cells."

Read the rest here"

Stem Cell Breakthrough: New Method For Creating Stem Cells

Dr. Nagy discovered a new method to create pluripotent stem cells (cells that can develop into most other cell types) without disrupting healthy genes. Dr. Nagy's method uses a novel wrapping procedure to deliver specific genes to reprogram cells into stem cells. Previous approaches required the use of viruses to deliver the required genes, a method that carries the risk of damaging the DNA. Dr. Nagy's method does not require viruses, and so overcomes a major hurdle for the future of safe, personalized stem cell therapies in humans.

"This research is a huge step forward on the path to new stem cell-based therapies and indicates that researchers at the Lunenfeld are at the leading edge of regenerative medicine," said Dr. Jim Woodgett, Director of Research for the Samuel Lunenfeld Research Institute of Mount Sinai Hospital. Regenerative medicine refers to enabling the human body to repair, replace, restore and regenerate its own damaged or diseased cells, tissues and organs.

PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS

Nagy lab

Canadian researchers have found what could be a new way to make embryonic-like stem cells

Stem Cell Research Blog

Researcher reprogrammed stem cell to help fight infertility

STEM CELL RESEARCH

Welcome to the Stem Cells Portal

Mount Sinai Hospital researcher makes stem cell breakthrough

Scientists have found a way to make an almost limitless supply of stem cells that could safely be used in patients while avoiding the ethical dilemma of destroying embryos

Courtesy of faz.net

Links:

(Toronto, ON, February 25, 2009) – In a study to be released on March 1, 2009, Mount Sinai Hospital's Dr. Andras Nagy discovered a new method of creating stem cells

Stem cell breakthrough ends ethical dilemma

Breakthrough makes lab-produced stem cells safer for humans

Canadian researchers have found what could be a new way to make embryonic-like stem cells, a discovery that could lead to cures for devastating conditions such as spinal cord injury and Parkinson's disease

A UK and Canadian team have manipulated human skin cells to act like embryonic stem cells without using viruses - making them safer for use in humans

Stammzellforschung weiter vor in ihrem Versuch, gewöhnliche Körperzellen komplett zu verjüngen und daraus das Rohmaterial für jeden beliebigen Gewebe- oder Organersatz zu züchten

Zwei Forschergruppen haben auf einem völlig neuen Weg Stammzellen gewonnen: Erstmals stellten sie aus Hautzellen eine Art embryonale Stammzellen her, die kein künstlich zugeführtes Gen mehr enthalten.

Als „Gentaxi“ nutzten sie jedoch keine Viren, die oftmals unkontrolliert in Zellen eingreifen, sondern ein kurzes Stück Erbmaterial, ein sogenanntes Transposon

First French human embryonic stem cells

Stem Cell and Cancer Biology, Cincinnati, OH, USA

Researchers expected to find at least 100,000
genes in the human body, but found only a fraction of that -- less than 30,000.

It's all in the epigenes

Epigenetics and human disease: translating basic biology into clinical applications

The Krembil Family Epigenetics Laboratory T

Embryo Stem Cell Biology

Professor Austin Smith is the Chair of
the Wellcome Trust Centre for Stem Cell Research.
Courtesy of bioc.cam.ac.uk

The main objective of this group is to characterise the cellular and molecular mechanisms governing the self-renewal and differentiation of multipotential embryo stem cells, of mouse, rat and human origin. Stem cells are defined by the ability both to produce identical daughter cells (self-renewal), and to produce progeny with more restricted fates (commitment and differentiation). This property of stem cells underpins growth and diversification during development and sustains homeostasis and repair processes throughout adult life.

Source:

Embryo Stem Cell Biology

Austin Smith is MRC Professor in Stem Cell Biology, at the University of Edinburgh

Animal-Human Hybrids and Chimeras For Stem Cell Research

What are stem cells?

KEEP YOUR HANDS OFF OUR OVARIES!

Screening embryos for autism could prevent eccentric geniuses like the physicist Paul Dirac from ever being born

Human Pluripotent Stem Cells without Cloning or Destroying Embryos

STEMCELL Technologies Launches Direct Distribution to Singapore

Share Your Views About Donating Eggs For Stem Cell Research

Stem Cell Science announces: Creation of authentic rat embryonic stem cells

Developing a technique for generation
of authentic embryonic stem (ES: 5.43, 0, 0%) cells
from rats.Courtesy of stemcell.taragana.net

Stem Cell Science plc has announced its groundbreaking research, developing a technique for generation of authentic embryonic stem (ES: 5.43, 0, 0%) cells from rats. It is an international research and development company focused on the commercial application of stem cell biology technologies for drug discovery and regenerative medicine research.
The research was published in the journal Cell is the first of its kind that demonstrates germ-line transmission from rat ES cells. It makes use of a technology licensed exclusively to SCS by the University of Edinburgh and developed by Professor Austin Smith and his team, now at Cambridge University. the new technique would be generating consistently appropriate and stable at ES cells, which can be used to create genetically modified animals for medical, academic and pharmaceutical research.
According to Dr Alastair Riddell, Chief Executive Officer of Stem Cell Sciences, the new technique would pave way for more effective drug discovery

Source:

Stem Cell Science announces creation of authentic rat embryonic stem cells

Scientists Cure Mice with Adult Stem Cells

Ukraine babies in stem cell probe

Stem Cell Alchemy Refined

Suzanne Somers: Stem Cell Master?

Everything we’ve learned about cancer can be summarized as ‘unpredictable and wildly varying’. To go into a tumour and think that if there’s a cancer that marker will always be there, I think that’s naive

First ever embryonic stem cell trial on spinal cord injury patients waiting for FDA approval

2009 Stem Cell Trendsetters in Neurology and Psychiatry

How Embryonic Stem Cell Lines are Made

Cerebral Palsy and Regenerative Medicine

Niehans' Stem Cell Therapy

Ashich Stem Cells's Blog Is Your Favorite Blog For Your Research

Stem cells in Ashich Blog

DIFFERENTIATION ARREST BY HYPOXIA

By: Qun Lin, Yi-Jang Lee and Zhong Yun

The stem cell niche is a unique tissue microenvironment that regulates the selfrenewal and differentiation of stem cells.Although several stromal cells and molecular pathways have been identified, the microenvironment of the stem cell niche
remains largely unclear. Recent evidence suggests that stem cells are localized in areas with low oxygen. We have hypothesized that hypoxia maintains the that hypoxia reversibly arrests preadipocytes in an undifferentiated state. Consistent with this observation, hypoxia maintains the expression of pref-1, a key stem/precursor cell gene that negatively regulates adipogenic differentiation. We further demonstrate that the hypoxia-inducible factor-1 (HIF-1) constitutes an important mechanism for the inhibition of adipogenic differentiation by hypoxia. Our findings suggest hypoxia in the stem cell niche is critical for the maintenance of the undifferentiated stem or precursor cell phenotype.

Source:
DIFFERENTIATION ARREST BY HYPOXIA

Leukemia stem cells (LSCs) comprise a functionally distinct subpopulation of leukemic cells with the ability to
self-renew extensively, and to initiate, sustain, or regenerate disease.

By:Piu Wong, Masayuki Iwasaki, Tim C.P. Somervaille, Chai Wai Eric So, and Michael L. Cleary1

Leukemia stem cells (LSCs) comprise a functionally distinct subpopulation of leukemic cells with the ability to self-renew extensively, and to initiate, sustain, or regenerate disease. In acute myeloid leukemia (AML), LSCs are generally considered to be rare upstream cells that arise out of the normal hematopoietic stem cell (HSC) or primitive progenitor compartments, and are organized in a hierarchy based on quantitative differences in their self-renewal potentials (Passegue and Weisman 2005).

Source:
stem cell potential leukemia MLL is an essential and rate-limiting ...

Cellular origin of cancer: dedifferentiation or stem cell maturation arrest?

Control of Cell Proliferation by Progress in Differentiation: Clues to Mechanisms of Aging, Cancer Causation and Therapy

Telomeres, stem cells, senescence, and cancer

Both Stroma and Stem Cell Factor Maintain Long-Term Growth of ELM Erythroleukemia Cells

Julius Friedrich Cohnheim was a pioneer of experimental pathology (Born 20 July 1839; died 1884 )

Julius Friedrich Cohnheim .
Photo courtesy of todayinsci.com

Cohnheim proposed the first great theory of cancer's origin, the theory of embryonal rests. He thought more germ cells are produced with a developing embryo than are needed to form any given part and that cancer's development involves this excess material.

Source:
Cohnheim in Todayinsci

FEWER RISKS, NEW HOPE:
THE REALITY OF BLASTOCYST TRANSFERS

Pathology, Wikipedia and Cohnheim

Julius Friedrich Cohnheim, a Prussin-born German pathologist

Adult Human Stem Cells & Cell-to-Cell Communication in Human Cancer

The mouse feeder cells were an important ingredient in the mix of culture materials required to keep stem cells in their undifferentiated "blank slate" state. Embryonic stem cells are capable of forming any of the 220 tissues and cells in the human body and, in culture, are constantly trying to migrate down different developmental pathways. Maintaining stock cultures in their undifferentiated state is critical.

Scientists rid stem cell culture of key animal cells

Differentiation at Biology News

Germ line stem cell differentiation in Drosophila requires gap junctions and proceeds via an intermediate state

It is a well-known curiosity that only a minority of cells within a tumour are typically responsible for tumour growth and development. >

Cancer stem cells: Differentiation block or developmental back-tracking?

Unlike most other structures in the adult brain, the hippocampus is able to produce new neurons (neurogenesis) throughout adult life as it contains a population of neural stem cells.

Microglia: The Enemy Within?

Phorbol Ester Effect on Differentiation of Human Myeloid Leukemia Cell Lines Blocked at Different Stages of Maturation

Cells That Go Back in Time

Dedifferentiated, programmable stem cells of monocytic origin, and their production and use

Stem Cells Forward and Back

WHITE PAPER: Alternative Sources of Pluripotent Stem CellS

Obtaining pluripotent stem cells without destroying human embryos

Induced dedifferentiation: A possible alternative to embryonic stem cell transplants

Stem-Cell Finesse Too Grotesque

Differentiation of Human Embryonal Carcinomas In vitro and In vivo

Regenerative Chemical Turns Muscle Cells Into Stem Cells

House of Lords - Stem Cell Research - Report

Stem Cells and Development

Reprogramming insights: in silico modeling suggests active dedifferentiation

Stem Cells from Differentiated Cells

By: Panagiotis A. Tsonis

A recent study by Chen et al. characterizes the small molecule reversine, a substituted purine analog, as a signal for the dedifferentiation of myotubes (formed from a myoblastic cell line) back into progenitor cells that can then differentiate, under appropriate conditions, into osteoblasts or adipocytes. The authors speculate that the process may involve protein kinases and that further work will identify the spe-cific kinase or other molecules to which reversine binds. This work is of extraordinary interest and may have landmark importance to regeneration research (i.e., reforming nerves, limbs, and organs) and clinical medicine.

Figure 1. Transdifferentiation in urodele limb
and lens regeneration and in a committed murine cell line.
Courtesy of molinterv.aspetjournals.org

Click on image to view larger version

Figure 1. Transdifferentiation in urodele limb and lens regeneration and in a committed murine cell line. After amputation of the newt limb (or tail) the intact terminally differentiated cells (such as, mesenchymal cells, myotubes, nervous tissue, bone, or cartilage) dedifferentiate by losing the characteristics of their origin. This dedifferentiation process produces the blastema cells, which then redifferentiate to reconstitute the lost limb. After lentectomy, the dorsal iris pigment epithelial cells lose their pigments and become dedifferentiated cells, which consequently regenerate a perfect lens. A small molecule, reversine, is able to induce dedifferentiation of myotubes formed by murine C2C12 cells. This dedifferentiation produces mesenchymal progenitor cells that are able to differentiate to different cell types, such as adipocytes and osteoblasts. According to this scheme, the reversine produced progenitor cells could be analogous to the blastema cells or other dedifferentiated cells used during regeneration in urodeles.

Source:

Stem Cells from Differentiated Cells

Reversine: an easy source of cells to regenerate tissues damaged by disease or injury.A chemical could switch adult cells from one type to another.

Chemists in California have found a synthetic molecule that seems to reprogramme adult cells to make them more like youthful ones. If the discovery pans out, it could provide an easy source of cells to regenerate tissues damaged by disease or injury.

Source:

Drug may give cells a fresh start

Stem Cells and Their Niches

Developmental Regulation of Hematopoiesis

Scientists have turned to the zebrafish for clues about congenital disease and cancer

Purdue developing less costly model for studying human disease

Progress towards cell-mediated gene transfer in zebrafish

Zebrafish Embryo Cells Remain Pluripotent and Germ-Line Competent for Multiple Passages in Culture

Production of zebrafish germ-line chimeras from embryo cell cultures

Zebrafish could become genetics 'lab rat' of choice

Germ at biology news

cell culture

Tumors are caricatures of the organ in which they arise

It is becoming apparent that tumors are caricatures of the organ in which they arise. The cancer cells in a tumor are arranged in a hierarchy and contain a minority population of cancer stem cells that drive tumor growth while the majority of the cancer cells are unable to do so. Normal stem cells and cancer stem cells maintain themselves through a process called self-renewal. The cancer stem cells are responsible for the growth and spread of tumors. Expansion of normal stem cells is under genetic constraints. Cancer stem cells have escaped these limitations on expansion resulting in expansion of the self-renewing cell populations. The goals of this meeting are to understand present the most current research into the cellular and molecular biology of cancer stem cells. Topics that will be explored in this meeting are the cancer stem cell niche, the cell of origin of cancer stem cells, the regulation of stem cell self-renewal in normal stem cells and cancer, and cancer stem cells as therapeutic targets.

Source:

STEM CELLS & CANCER

Solid tumors are caricatures of the organ in which they arise

Cancer and Stem Cells

The Biology of Cancer Stem Cells - Annual Review of Cell and ..

In search of a stem cell hierarchy in the human breast

Cancer Stem Cells and Differentiation Therapy

Stewart Sell, M.D & Stem cells

Stem Cells Stewart Sell Hepatocytes following ...

Sell S. Stem cells in hepatocarcinogenesis

Stem Cell Reviews. Editor-in-Chief:. Stewart Sell

Human myeloid leukemias provide models of maturation arrest and differentiation therapy of cancer

Stem cells are essential elements of rapidly turning-over cell populations

Adult Stem Cells - Google Books Result

Joe W. Grisham

Hepatic stem cells are activated after liver damage and have a critical role in tissue homeostasis and repair

Rebuilding the liver with adult hepatic and embryonic stem cells

Spontaneous differentiation of mouse embryonic stem cells in vitro

Cells may help researchers in skin and hair therapies

Islam on Stem Cell Research

News: UCI seeks stem-cell grant

Adult Stem Cell Awareness

Churched Stem Cells

Why Pro-Stem-Cell Research Is Not Prolife

The Catholic Church's Teaching on Stem-Cell Research

The stem-cell movement has become political

Cloning, stem cells, and religion?

The Scientist : Calif. stem cell grant raises concerns

Stem Cells In New Jersey

PML-RARA-Targeted DNA Vaccine Induces Protective Immunity in a Mouse Model of Leukemia

Textbook of Malignant Haematology - Google Books Result

All trans retinoic acid in acute promyelocytic leukemia

Retinoic Acid and Arsenic for Treating Acute Promyelocytic Leukemia

Resistance to All-Trans Retinoic Acid (ATRA) Therapy in Relapsing ...

Molecular Target-Based Treatment of Human Cancer

Retinoic Acid and Arsenic Synergize to Eradicate Leukemic Cells

Gary Gilliland
& Stem cells

Targeting NF-B in hematologic malignancies

Identification of a Cancer Stem Cell in Human Brain Tumors

Cancer Stem Cells: At the Headwaters of Tumor Development - Annual

Stem cell biology and the cellular pathways of carcinogenesis

Glioblastomas (GBM) are among the most biologically aggressive and therapeutically challenging cancers

Brain Tumor Stem Cells: New Targets for Clinical Treatments?

Brain Tumor Stem Cell-targeted Gene Therapy

Brain tumor stem cells: identification and concepts

Comprehensive Brain Tumor Program & Stem cells

Differentiation profile of brain tumor stem cells: a comparative study with neural stem cells

Brain tumours are typically comprised of morphologically diverse cells

Brain tumors may originate with neural stem cells

Stem Cell Background

Neural Stem Cell (NSC) research

Royan Institute (Tehran/Iran):Department of Stem Cells

Members:
Narges Zare Mehrjardi (Neuroscience PhD student)
Maryam Hatami (Cell & Molecular Biology MSc)
Sahar Kiani (Physiology PhD student)

This group has been committed to exploring the biology and therapeutic potential of stem cells for the treatment of neurological disorders. For this purpose we focused on differentiation, transplantation, tissue engineering and signaling of neuron derived stem cell. Recently we transplanted differentiated neural tube from human embryonic stem cell to spinal cord injured rat.

Stem cell research will increase our understanding of the nervous system and may allow us to develop treatments for currently incurable brain and spinal cord diseases and injuries. In addition, the stem cells should be used for stem cell research aimed at the detailed study of mechanisms of neural differentiation, transdifferentiation, genetic and environmental signals that direct specialization of the cells into particular cell types. Breakthrough studies have recently rejected the long-standing belief that neuronal tissue is incapable of regeneration.

The successful engraftment of neural stem cells following implantation into the brain of rodent models has demonstrated the potential of this cell type. The development of neural stem cell biology requires specific research tools that allow the isolation, expansion and characterization of this stem cell type. In addition, the availability of reagents for the characterization of progenitor cells is critical for the analysis of their therapeutic potential. Neural stem cells can be derived from embryonic sources, or from specialized niches in the adult nervous system. Because they have the capacity to generate all of the multiple cell types found in the brain and spinal cord, neural stem cells have the potential to repair tissue injured by trauma or disease.

Source:
Royan Institute & Nerves System

hESC-derived neurons. Courtesy of
royaninstitute.org

The discovery that neurons, astrocytes and oligodendrocytes arise from neural stem cells located in specific regions of the central nervous system has important clinical applications for the treatment of life-threatening, neuronal diseases, including Parkinson’s Disease.

Neural Cells

Royan Institute

Stem Cells

Beneficiary of Adult Stem Cell Treatment for Parkinson's Disease

The lineage steps in the development of the mammalian brain from totipotent embryonic stem (blastocyst) cells to neural stem cells

Stem Cells: What Are They?

MNI Study Identifies New Source of Stem Cells

Stem cell plasticity revisited: CXCR4-positive cells expressing mRNA for early muscle, liver and neural cells 'hide out' in the bone marrow

SAMUEL WEISS & Stem cells

Nestin-expressing neural stem cells identified in the scar ...

Tumours and stem cells

Cytokine regulation of neural stem cell fate in the developing ...

New hope in neural transplants

Isolation Of Stem Cells May Be A Treatment For Hearing Loss

UMBILICAL CORD BLOOD STEM CELL RESEARCH

Safety Issues in Stem Cell Transplants

Stem Cell Genomics Project (aka Gene Expression Project) Update

HOW STEM CELLS FORM NEURONS

All stem cells are unspecialized (not involved with a specific area) cells

New hope for stroke patients: mobilization of endogenous stem cells

STEM CELL RESEARCH AND THERAPY: HOW CLOSE ARE WE, REALLY?

Stem Cell Research News

Science's Next Frontiers

Sonic "Hedgehog" gives stem cells a boost

Molecular mechanisms of stem-cell identity and fate

Stem cells track down tumour

Links:

Solimena Group & Universtaetsklinikum Dresden

Michele Solimena - Regulated secretion and signal transduction in pancreatic β-cells and neurons

C Y- 3 (MSY3) Anna Natalia Grzyb

The native cellular microenvironment of stem cells is termed the stem cell niche

Human embryonic stem cell microenvironment suppresses the tumorigenic phenotype of aggressive cancer cells

Stem Cell Interactions with their Microenvironmental Niche (X1)

Stem cell microenvironment reverses malignant melanoma

Cell Patterning Chip for Controlling the Stem Cell Microenvironment

Microenvironment created by stromal cells is essential for a rapid ...

Microenvironment drives the endothelial or neural fate of ...

Preparation of Mouse Embryonic Fibroblast Feeder Cells for Human Embryonic Stem Cell Culture

Preparation of Human Foreskin Fibroblasts for Human Embryonic Stem Cell Culture

A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells

Derivation of human embryonic stem cell lines from single cells of 4-cell stage embryos: be aware of the risks

A morphological and chromosomal study of blastocysts developing

Good manufacturing practice and clinical-grade human embryonic stem cell lines

Pluripotent stem cell lines

Field Leaps Forward With New Stem Cell Advances

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Human neural crest cells display molecular and phenotypic hallmarks of stem cells

Regulation of Self-Renewal and Pluripotency by Sox2 in Human Embryonic Stem Cells

Reciprocal Transcriptional Regulation of Pou5f1 and Sox2 via the Oct4/Sox2 Complex in Embryonic Stem Cells

Zfp206 Is a Transcription Factor That Controls Pluripotency of Embryonic Stem Cells

Oct4 and Sox2 Directly Regulate Expression of Another Pluripotency Transcription Factor, Zfp206, in Embryonic Stem Cells

Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles

Mammalian neuronal differentiation: early expression of a neuronal phenotype from mouse neural crest cells

Transcriptional Regulation of Nanog by OCT4 and SOX2

by Terry Devitt
What are embryonic stem cells?

Guidelines for Human Embryonic Stem Cell Research

a great deal of misinformation encircling the public discussion of embryonic stem cell research (ESCR)

Many proponents of embryonic stem cell research claim that ...

Is high-tech cannibalism the way to economic growth?

Cannibalistic Signals Help Mammalian Embryos Develop Normally

Medical Cannibalism.(stem cell research and ethics)(Brief Article)

http://www.articlecity.com/articles/politics_and_government/article_472.shtml

Biotech Cannibalism

Cannibalism:
Eyewitness accounts from inside the booming trade in fetal body parts

Pope Condemns Euthanasia and Embryonic Stem Cell Research as Barbarism

Stem Cell Research and Frankenstein

Frankenstein's Dog and Stem Cells

Can Stem Cells Reverse Heart-Attack Damage?

Human Embryonic Stem Cells Assemble and Fulfill Their Developmental Destiny

Stem cell fakery called 'criminal act in academia'

Stem cell tricks hint at baldness cures

Just what can adult stem cells do?

Observing Stem Cell Growth and Viability

Human-Serum Matrix Supports Undifferentiated Growth of Human Embryonic Stem Cells

Claudia Castillo gets windpipe tailor-made from her own stem cells

A woman has been given a new section of windpipe created from her own stem cells in an operation that could revolutionise surgery.
Claudia Castillo, 30, who lives in Barcelona, has become the first person to be given a whole organ tailor-made for her in laboratories across Europe.
A graft from a donor was used, but because it has been imbued with Ms Castillo’s own cells, there is no sign that her body will reject the organ.

Source:

A woman has been given a new section of windpipe created from her own stem cells in an operation that could revolutionise surgery

Links:

Ms Castillo's cells, grown at the University of Bristol from stem-cell samples from her bone marrow to create the cartilage

Claudia Castillo gets windpipe tailor-made from her own stem cells

Doctors transplant windpipe with stem cells

Stem cells show potential to repair lungs in mice

Stem cells

Klon ve Klonlama Nedir?

Tek hücreli insan zigotunun 100 trilyon hücreli

Scientists may have found a safer way of giving a flake of skin the biologically alchemical powers of embryonic stem cells

Scientists may have found a safer way of giving a flake of skin

More Links:

Stem Cells Without Embryonic Death

Human embryonic stem cells (hESCs) are valued for their potential to turn into most cell types of the body. With continuing research, they hold strong promise in the field of cell and tissue engineering

Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium

Stammzellen: Was können wir wollen

Kultivierung embryonaler Stammzellen

Embryonale Stammzellen auf dem Weg zur Massenkultur

Die Rolle der Stammzellen im adulten Gehirn

Aus Stammzellen erzeugtes Organ erfolgreich transplantiert

Leukämie - Was kann ein Einzelner tun?

Selenium Induces Improvement of Stem Cell Behaviors

Iran invests $2.5b in stem cell research

Iran in the forefront when it comes to stem cell research

Genetic manipulation of embryonic stem cells

Stem Cells Feed Brain Tumors

Embryonic Stem Cell-Derived Dendritic Cells Expressing Glypican-3, a Recently Identified O

Links:

Transcriptional Regulation of the Stem Cell Leukemia Gene by PU.1 ...

Stem Cell Research Basics: Early Stem Cells

Inner cell mass

Stem Cell Basics

Fate of the inner cell mass in mouse embryos as studied by microinjection of lineage trace

Isolation and culture of inner cell mass cells from human blastocysts

Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines

Tetraploid Embryonic Stem Cells Contribute to the Inner Cell Mass ...

Maintenance of the Inner Cell Mass in Human Blastocysts from Fragmented Embryos

XYClone for Inner Cell Mass Isolation

Isolation of inner cell mass for the establishment of human embryonic stem cell (hESC) lines

Efficient isolation inner cell mass from blastocysts by improved microsurgical technique

cyst with Inner Cell Mass

B-myb Is Required for Inner Cell Mass Formation at an Early Stage of Development

Not only inner cell mass cell nuclei but also trophectoderm nuclei of mouse blastocysts have a developmental totipotency

Oct-4 expression in inner cell mass and trophectoderm of human blastocysts

Deletion of beta 1 integrins in mice results in inner cell mass failure

BrainStorm Cell Therapeutics Elects Moshe Lion to Its Board of Directors

The Potential of Marrow Stromal Cells for the Cell-Based Therapeutic treatment of ocular diseases

The recent discovery of the so-called ‘induced pluripotent (iPS)’ cells

Stem Cells & Stem Cell Therapy

Stem cells at root of antlers' branching

Scientists Use Monkey Clones to Extract Stem Cells

Transplantation Of Monkey Embryonic Stem Cells Reverses Parkinson Disease In Primates

Monkey embryonic stem cells a world first

Stem Cells From Monkey Teeth Can Stimulate Growth And Generation Of Brain Cells

Monkey stem cells cloned

Monkey Teeth Stem Cells Hold Promise For Brain Cells

Stem Cell Advance in Cloned Monkey Embryos Renews Human Cloning Debate

Researchers Derive Stem Cells From Monkeys

Squirrels may know a cure for cancer!

Frog provides insight into making specialised cells from stem cells

Frogs Can Play Key Role In Stem Cell Research

Scientists Grow New Frog Eyes From Stem Cells

Antibodies in Lab: Human Embryonic Stem Cell Marker Antibody Panel Plus

African Claw Frog Helps Stem Cell Research

Are Stem Cells a Regenerative Fountain of Youth ?

Stem cell experts offer guide to fight "snake oil"

Stem cell therapy or snake oil?

Adult Stem Cells: Snake Oil?

Stem Cells: A Pathway Through the Maze

Stem Cells Bring New Strategies for Developing Replacement Neurons

Stem Cells Did you know you had any?

During embryogenesis, a single fertilized oocyte gives rise to a multicellular organism

Harvard Stem cell Scientists on their way to reshape medicine for disease producing cells

Lineage Restriction in Stem Cells

Embryonic stem cell research and therapy

Printers aid stem cell research

Core transcriptional network of embryonic stem cells

Modeling the core transcriptional machinery
in embryonic stem cells
.Computational Biology & Biological Physics Department of Theoretical Physics, Lund University
. The core transcriptional network of the embryonic stem cell
.Generics about switches and network motifs ynamical models
.Results: (I) Switch properties (II) Target gene regulation
.What about noise?
.Other stem cell systems ....

Measurements:

*Several “single gene” experiments have identified
OCT4, SOX2 and NANOG as key players
*Chip-ChiP experiments
Human: Boyer LA et al., Cell 122:947-956 (2005)
Mouse: Loh Y-H et al., Nat Gen 38:431-440 (2006)
*Microarray profiling
Mouse: Ivanova N et al., Nature 442:533-538 (2006)

Epigenomics & Sequencing

GeneExpression Systems, Inc. is a biotech company that develops and markets differential gene profiling technology for down stream study of gene expression, regulation, diagnostic, predictive toxicology and therapeutic purposes. The company is situated in the Greater Boston area biotech hub and commercializes proprietary gene expression kits, services and technologies to accelerate and enhance the discovery of new markers.

GeneExpression Systems, Inc. is a biotech company

Global distribution of negative cofactor 2 subunit-α on human promoters

Eine Schlüsseltechnik
für dessen Dechiffrierung ist die Chromatin-Immunpräzipitation
(ChIP)

Das Genom der meisten Zellen in Metazoen ist identisch, während Phänotyp und Funktion sich dramatisch unterscheiden. Das dahinter stehende Programm der Zellen ist in ihrem Epigenom gespeichert. Eine Schlüsseltechnik
für dessen Dechiffrierung ist die Chromatin-Immunpräzipitation(ChIP). Für deren Analyse wurden jetzt neue effiziente Parallelsequenziermethoden(ChIPseq) entwickelt, die herkömmlichen Mikroarrays Konkurrenz machen werden.

Source:

Die Entschlüsselung des Epigenoms

Broad Institute Awarded Major Grant to Bolster Epigenomics Research

Mapping polycomb complexes in human and mouse embryonic stem cells

The first five years of the Wnt targetome

ChIP-seq: welcome to the new frontier

Chip-Sequencing

Chip sequencing. Courtesy of wikimedia.org

Dopaminergic neuron differentiation
from human embryonic stem cells”

Dr. Mahendra Rao. Courtesy of cdb.riken.jp

Dr. Mahendra Rao

The adult brain has a limited capacity to regenerate new neurons, particularly those of the substantia niagra,spinal cord, and cortex. In contrast, glial cells can be regenerated at low numbers throughout the adult life span and the numbers generated increase substantially after injury. Despite the ability of the adult brain to undergo neurogenesis and glial genesis, a deficiency in adequate neural replacement after cell death by disease or injury results in a variety of debilitating neurological conditions. In particular, neurological disorders associated with
loss of specific neural cell types, such as oligodenocyctes or dopaminergic neurons, has lead to a search for disease treatments including mobilization of endogenous stem/precursor cells to generate suitable replacement cells, development of methods to deliver or induce secretion of trophic molecules to prevent cell loss, and
transplantation of cells for localized repair.

Source:

Dopaminergic neuron differentiation

Links:

Mahendra Rao: Stem Cell Biology

Mahendra Rao:

Stem-cell biologist Mahendra Rao is embarking on a dual career combining a top job in industry and basic academic researchfont>

Mahendra Rao:

Embryonic stem cells possess two properties that make them especially well suited for cell therapy. First, because embryonic stem cells are obtained from early blastocysts, they are at a very early developmental stage, and retain the flexibility to become any one of the more than 200 cell types that make up the human body

Given the right combination of signals, embryonic stem cells will develop into mature cells that can function as neurons, muscles, bone, blood or other needed cell types. Stem cells with such flexibility are described as "pluripotent," to indicate their high potential to differentiate into a wide variety of cell types.

Source:

Human Embryonic Stem Cells

Links:

Stammzellen-Import: Pro und Contra

Stammzellen gegen Schlaganfall

Stammzellen, die Joker-Zellen in Ihrem Leben!

War Gott der erste Stammzellenforscher?

War Gott der erste Stammzellenforscher?

Stammzellen beschleunigen das Leberwachstum

Scientist: Stem cells could end animal testing

Cell counting and classification

UWA scientist says stem cells find is just the start

Stem Cell Summit

WiCell

UW–Madison has numerous educational and training programs related to stem cell biology, developmental biology and regenerative medicine

Post-doctoral Fellow Position, Institute of Medical Biology (IMB), Singapore

To provide students with information on the latest developments in animal and human stem cell research

European Stem Cells & Regenerative Medicine Congress, May 12-14, London

Source:

Stem cells & Congress, 2009

Texas Heart Institute provides postdoctoral training opportunities

Stem cell research in India found a sign of relief for stroke patients

Gene Keeps Stem Cells Healthy

Welcome to the 3rd annual Stem Cells World Congress. This year's event will be held in beautiful Palm Springs, California - definitely the place to be in January!

Stem Cells for Brain Injury Recovery?

2009 Stem Cell Trendsetters in Neurology and Psychiatry

Tutorial 2: ChIP-Seq Data Analysis

ChIP-seq/Methylation/Epigenetics

ChIPSeq and Epigenetics

ChIP-Seq Data Analysis

Design and analysis of ChIP-seq experiments for DNA-binding proteins

Massive gene screening points way to more effective chemotherapy

The Latest Technology May Not Have Transformed Your Health, But It Has Changed Science
Dr. Michael White, a biochemist and a postdoctoral fellow in the Department of Genetics and the Center for Genome Sciences at the Washington University School of Medicine

What is that stretch of DNA? ChIP-seq
With the latest DNA sequencing technology, you can dispense with building a DNA chip. You no longer need to make probes, put fluorescent chemicals on your DNA, and see which probes your DNA sample sticks to. Instead, you just take your DNA sample sequence it - you can now find out directly just exactly what DNA is in that small, clear drop of liquid

Source:

The Latest Technology May Not Have Transformed Your Health, But It Has Changed Science

Next-generation sequencing: applications beyond genomes

Cashing in Your Chips to Advance Discovery

Anduril components for ChIP-Seq

Next-generation DNA sequencing

Gene Regulation – It’s All In The DNA

Can iPS Replace The Need for Embryonic Stem Cells?

Adult Stem Cells From Monkey Teeth Stimulate Generation Of Brain Cells

Things You Should Know About Stem Cell Research

The Nucleosome Roadblock On The Path To Understanding Gene Expression

Finding a New Gene Behind Language Disorders

Those Dang Transcription Factors

Chromosomes Are So 20th Century - Male Genes Really Determine Baby Gender, Says Study

What Next Generation DNA Sequencing Means For You

ChIP-Seq Data Analysis

Motor Protein That Rewinds DNA

Site-Seeing by Sequencing

A reduction in cost of massive DNA analysis by factor of 30 is big news.

ChIP-Seq for the first human genome-wide mapping

MISMATCH ENRICHMENT METHOD FOR DISCOVERING HUMAN SNPS

From DNA Sequence to Chromatin Dynamics: Computational Analysis of ...

DNA methylation and chromatin remodeling

On microRNA and the Need for Exploratory Experimentation in Post ...

CREATING STEM CELLS FOR RESEARCH. Information & Photos From: genetics.utah.edu

Source:

CREATING STEM CELLS FOR RESEARCH

Courtesy of genetics.utah.edu

Modelling, Simulation and Visualisation of Stem Cell Behaviour

First functional stem-cell niche model created by Stanford scientists

Team finds new mechanism of gene control

Mechanism That Controls Activation Of Stem Cells During Hair Regeneration Identified

Practical Modeling Concepts for Connective Tissue Stem Cell and Progenitor Compartment Kinetics

Courtesy of nature.com

Source:

Metarandom states push cell fate
Natalie DeWitt

Mathematical modeling of stem cells: A complexity primer for the ...

Elevating the levels of Sox2 in embryonal carcinoma cells and embryonic stem cells inhibits the expression of Sox2:Oct-3/4 target genes

Courtesy of nature.com

Source:

Parsing pluripotency: the role of Sox2
Natalie DeWitt

Gene Directs Stem Cells To Build The Heart

The complex choreograph of cells that starts with the fertilized egg involves many signals telling cells

Passage to Global Stem Cells

No human embryos created or destroyed: Embryonic Stem Cells Created from Skin Cell

Like nanotechnology, particle physics, and alchemy before it, stem cell research has become the latest media-attention buzzword scientists dream about.

Ultra High Throughput Sequencing

Using a massively parallel sequencing approach, the Illumina Genome Analyzer (GA) can generate billions of bases of high quality sequence data in a single run. The system uses Solexa sequencing technology and novel reversible terminator chemistry, optimized to achieve unprecedented levels of cost effectiveness and throughput.

DNA Technologies, Solexa Test

Souce:

Ultra High Throughput Sequencing

ChIP-Grade Protein G Magnetic Beads

Next-generation sequencing transforms today's biology

The latest next-generation sequencing
instruments can generate as much data in
24 h as several hundred Sanger-type DNA
capillary sequencers, but are operated by
a single person. Courtesy of nature.com

Welcome to the New World of Wrap-Speed DNA Sequecing

Is your lab's approach relies on traditional sequencing methods? If yes, please be aware of the new technology. You know exactly that your traditional used sequecing methods yields you not so much short stretches of DNA sequence at a time! Am I right? Do you agree with me? For instance, it yields a maximum of 96 short stretches of DNA sequence at a time.But there are many groups who use the "next-genration" Solexa sequencing platform from Illumina, Inc., which produces tens of millions of DNA sequences in a single run.Researchers agree that Solexa and two competing systems (the Roche (454) GS FLX sequencer and the SOLiD sequencer from Applied Biosystems) represent a breakthrough in sequencing that is speeding the pace of discovery, making it feasible for researchers to conduct experiments once considered too expensive or simply impossible.

Next-generation systems also analyze fragments of DNA but step up the process by multiplying the number of sequencing reactions that occur on each piece of template DNA, yielding vastly more data.Solexa produces shorter DNA sequence reads than traditional methods of sequencing, but many more of them-up to 50 million sequences in a single tow-day run. 454 and SOLiD use different teachniques of sequencing, but likewise produce large amounts of data.Joe DeRisi, an HHMI investigator researching malaaria and emerging viral diseases at the Univerity of California, San Francisco, gives the example of a fragment of a novel virus discovered in his lab a couple of years ago." This virus was very different than anything that ever been published before," he says. " A very talented postdoc threw every thing but the kitchen sink at this project, trying to sequence complete copy of the virus' genome and was unable to do so." The DeRisi team was stumped. Last year, they began working with the Solexa sequencer and decided to revisit the problem. They attempted to sequence the whole pool of DNA fromwhich the virus fragments was isolated." After a sigle run, we had recovered the entire genome in one go. In tow days we were able to accomplish in totality what we couldn't in over a year of hard trying," says DeRisi, whose team is preparing to publish their discovery. DeRisi points out that the new technologies are not a replacement for traditional sequencing methods, which are ideal for sequencing a very specific piece of DNA. But because of the ability of the new technologies to tackle large projects or complex mixtures of DNA, he and other HHMI researchers have great expectations.

Cancer researcher Bert Vogelstein, an HHMI investigator at Johns Hopkins Universsity, can now go through millions of gens to find the rare, tumor-specific ones that provide clues about the origins of disease-and about targets for therapy or cancer tests." Before, it was just too expenisve to study a lot of patients, but now we can," Vogelstein says. The technology isn't without its challenges, however. For example, the new systems spew out data with the force of afire hose. HHMI investigator Greg Hannon, whose team uses the Solexa instrument at Cold Spring Harbor Laboratory to study gene regulation, says it produces a terabyte, or one trillion bytes, or raw data. That's more sttarted filling hard drives with the data and "transferring" the information by foot to cars for delivery to a data center. he calls this a "sneaker protocol." That's sneaker as in shoes.

Internet Link:

Source:

Next-generation systems also analyze fragments of DNA

Applications of next generation sequencing

Polymorphism Discovery in Next-Generation Sequence Data

Next-generation sequencing formats

Next-generation DNA sequencing technologies such as Illumina's Solexa platform

Capillary Sequencing at The Gene Pool

The secret behind fast assembly of Next Generation Sequencing data

Next-Generation Sequencing Informatics

HHMI has purchased 15 of $ 500,000 Solexa sequencers for its investigators, and its investment in next-generation sequencing equipement is already playing dividends.

Next-Generation New Sequencing Technologies

DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection. Over the past three years, massively parallel DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing by over two orders of magnitude, and democratizing the field by putting the sequencing capacity of a major genome center in the hands of individual investigators.

Link & source:

Next-generation DNA sequencing by Jay Shendure & Hanlee Ji

Jay Shendure Assistant Professor of Genome Sciences:gs.washington.edu

The Shendure Lab - Laboratory of Jay A. Shendure M.D., Ph.D

Special Topics in Genomics

A new generation of technologies is poised to reduce DNA sequencing costs by several orders of magnitude

The ChIP'ing News

Diagrammatic representation of the chromatin immunoprecipitation (ChIP) procedure

Chromatin Immunoprecipitation (ChIP) Assay Kit

ChIP Assays and Components from IMGENEX: QuikChIP™ Certified Antibodies

Chromatin Immunoprecipitation (ChIP) Assays

Chromatin Immunoprecipitation chapter, Protein Interaction guide ...

Chromatin Immunoprecipitation (ChIP) Assay (PROT11)

Name of Videos from left to right:
1)Umbilical Cord Stem Cells
2)Stem Cell Video

Embryonic stem cells are the body's master cells, giving rise to all of the tissues in the body, and iPS cells, made using genes to transform ordinary cells, appear to have similar powers

Research shows that stem-like cells from circulating blood can restore function in rats with stroke damage

Induced pluripotent stem cell

Generation of germline-competent induced pluripotent stem cells

Patient-derived induced stem cells retain disease traits

Transplantation of neural stem cells modulates apolipoprotein E expression in a rat model of stroke

Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in embryonic stem cells

Plasma endothelin-1 levels after stem cell transplantation

Heparin promotes the growth of human embryonic stem cells in a defined serum-free medium

Human Stem Cell Factor (SCF) Is a Heparin-Binding Cytokine

UK Sugar Study Is Sweetener For Stem Cell Science

Sugar in the brain

Retinal Stem Cells in the Adult Mammalian Eye

Alcohol, Neural Stem Cells, and Adult Neurogenesis

China to Build Stem Cell Complex

Targeting Cancer Stem Cells with Phytochemicals

Stem cells in prostate cancer: resolving the castrate-resistant conundrum and implications for hormonal therapy

Immune System Series The Stem Cell

Sequencing the Sheep Genome

Working paper prepared by: Hutton Oddy, John McEwan, Brian Dalrymple, Jill
Maddox, Frank Nicholas, Chris Warkup and Alex Clop. 23 November 2006

The sheep genomics community is keen to sequence the sheep genome. This will facilitate research to improve the productivity of sheep and the health of the environments in which sheep are managed. It will also facilitate research using
sheep as a model for studies of human physiology and disease. Outcomes of the sequencing strategy proposed, in addition to sheep specific genomic sequence,include at least 1.4 million ordered SNPs for use in sheep genetic improvement,and information that will assist the assembly of the bovine genome.

Sequencing the Sheep Genome Working paper prepared by: Hutton Oddy ...

Pyrosequencing Inventor Building Mini Sequencer That Will Cost Fraction of 454's GS20

Longer Read Lengths on the Roche-454 GS20

Sheep Genomics Consortium Develops SNP Chip with Illumina

Second Generation Sequencing Technology(Polony Cyclic Sequencing by Synthesis)

The Genome Analyzer. Courtesy of illumina.com

Illumina introduces new products and services

The Genome Analyzer, Illumina

454 Sequencing accurately detects HIV tropism in patient samples

Colony Polymerase Chain Reaction of Stably Transfected Trypanosoma ...

Yeast Colony PCR

Polony is a contraction of "polymerase colony," a small colony of DNA

Sequence-Tagged Sites (STS)

Sequence-tagged sites and low-cost DNA markers for rice

Sequence-tagged sites (STSs) are short stretches of DNA sequence, usually a few hundred bases long

Molecular Cloning c. Creation of a DNA library

DNA Sequencing FAQ

DNA Library

How Genes Work DNA Structure and Function

Dolly and the Clone Wars

Computational Biology Lecture #5: Sequencing

Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle,

Principle of Pyrosequencing Technology

Pyrosequencing

Pyrosequencing: A Simple Method for Accurate Genotyping

PyroMark™Q24 System.Courtesy of pyrosequencing.com

PyroMark Vacuum Prep Workstation.Courtesy of pyrosequencing.com

PyroMark Vacuum Prep Workstation

Pyro Q-CpG can be performed
on any Pyrosequencing instrument, including .Courtesy of pyrosequencing.com

Pyro Q-CpG Software has been
developed to address applications that
benefit from high-quality and high-throughput
analysis of DNA methylation..Courtesy of pyrosequencing.com

Pyrosequencing technology: complete solutions for accurate genetic determination in clinical research

Quantify DNA methylation in a flash, like never before

Pyrosequencing, a High-Throughput Method for Detecting Single Nucleotide Polymorphisms

The advent of 'high-throughput sequencing by synthesis' platforms, such as the Roche FLX

Pyrosequencing (Roche platform) involves the use of a pyrophosphate molecule, released following nucleotide incorporation by DNA polymerase, to propagate reactions that ultimately produce light.

Sequencing chemistry

Pyrosequencing (Roche platform) involves the use of a pyrophosphate molecule, released following nucleotide incorporation by DNA polymerase, to propagate
reactions that ultimately produce light. Illumina’s sequencing-by-synthesis involves the use of four differently labelled fluorescent nucleotides that have their 3’-OH groups chemically inactivated to ensure only a single base is incorporated per cycle. Each base incorporation cycle is followed by an imaging step to identify the base incorporated, and a chemical stepthat removes the fluorescent group and eblocks the 3’ end for the next base incorporation cycle. The SOLiD sequencer (Applied Biosystems) uses a ligation-based sequencing process that starts by annealing a universal sequencing primer that is complementary to the SOLiD specific adaptors on the library fragments.

Source:

Roche (454) FLX Genome Sequencer

Illumina /Solexa Genome Analyzer II.
Courtesy of biotech.iastate.edu

Next-Gen Sequencing, Iowa State University researchers

Abstract for Genomic Cloning Technical Manual

Genome Sequencer FLX System

Genome Sequencer 20 System

Porcine gene discovery by normalized cDNA-library sequencing and ...

A pig multi-tissue normalised cDNA library: large-scale sequencing, cluster analysis and 9K micro-array resource generation

Generation of a bovine oocyte cDNA library and microarray

From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing

cDNA LIBRARY TITRE

Tearless Onion.Courtesy of imageshack.us

Silencing Onion Lachrymatory Factor Synthase Causes a Significant Change in the Sulfur Secondary Metabolite Profile

Onions produce the chemical irritant known as syn-propanethial-S-oxide

Why does chopping an onion make you cry?

Cell cultures as models for studying fish nutrition and development

Can cell cultures from fish be used to study obesity in humans?

The creepy solution to the stem-cell debate

Stem cells. Courtesy of viacord.com

Stem cells. Courtesy of virtualrosario.com

Scientist: stem cells could end animal testing

Médicos españoles usan por primera vez células madre de grasa abdominal para reconstruir la mama de pacientes de cáncer

LAS CÉLULAS MADRE

Células madre e hipocresía

Crean células madre sin dañar al embrión

El mundo de las células madre

Científicos crean roedor 'interespecie' empleando células madre de embrión

Several live Chinese Hamster Ovary (CHO) cells in vitro

Células Madre, Video

CELULAS MADRE BOLIVIA. Courtesy of celulasmadrebolivia.com

Celulas Madre Bolivia

Professor Ivan Bertoncello leads the Adult Lung Stem Cell Laboratory at the Australian Stem Cell Centre and is also responsible for the development and co-ordination of training programs in stem cell technologies for the Australian Stem Cell Centre

Professor Ivan Bertoncello. Courtesy of stemcellcentre.edu.au

Associate Professor Ivan Bertoncello leads the Adult Lung Stem Cell Laboratory at the Australian Stem Cell Centre and is also responsible for the development and co-ordination of training programs in stem cell technologies for the Australian Stem Cell Centre. He holds honorary academic appointments in the Department of Anatomy and Cell Biology, Monash University, and the Department of Pathology, University of Melbourne, and is a former Fellow of the Alexander von Humboldt Foundation (Germany). Associate Professor Bertoncello’s pioneering work in the development of Rhodamine 123 and Hoechst 33342 dye-based cell separative strategies for haemopoietic stem cell isolation, and his laboratory’s definitive study of haemopoietic stem cell cycling and turnover has provided the field with powerful tools for the analysis of haemopoietic stem cells leading to a re-evaluation of models of haemopoietic stem cell organization and function.

Associate Professor Ivan Bertoncello leads the Adult Lung Stem Cell Laboratory at the Australian Stem Cell Centre

The ASCC offers laboratory-based training courses to investigators wanting to learn how to isolate, maintain, propagate, manipulate, differentiate and analyse embryonic and adult stem cells

the highest quality in the stem cell field, in order to discover and ultimately commercialise new therapies for human disease

Developmental Biology Laboratory and how multipotent adult stem cells (ASCs) such as mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) are developed

Stem Cell Expansion Laboratory

Tissue Repair

Overview
Refining the human embryonic stem cell phenotype
Thus far, our research, has demonstrated that we can isolate viable pluripotent (Oct-4+) human ES cells from a heterogenous mixture of cells by selecting for two surface markers. This is important as this methodology provides a facile means for the separation of Oct-4+ cells from Oct-4– human ES cells without insertion of selectable markers. We have transcriptionally profiled subpopulations of hESC delineated using these cell surface markers. Current projects focus on gaining a better understanding of both the maintenance of the stem cell state and the decisions hESC make when becoming committed to a specific lineage.

Source:

Human Embryonic Stem Cell Technology

Professor Richard Boyd Director, Monash Immunology and Stem Cell Laboratories

Genomic and Proteomic Approaches to Complex Heart, Lung, Blood, & Sleep Disorders

The Jackson Lab, Current courses, meetings, and workshops

MDI & Courses and Conferences 2009

MAPC Training Program NHLBI-UMN MAPC Training Program

hES cell culture is generally considered more difficult than mouse ES cell culture. Mouse ES cells are often cultured on feeders made of immortal MEF cell lines, while hES cells need to be cultured on primary MEF cells. We will teach you to derive primary MEF cells in this course. hES cells are more difficult than mouse ES cells to culture, recover from a thaw, or passage. If you have mouse ES cell experience, but no hES cell experience, this course will offer you a lot of new information and technical training vital to the successful propagation of your hES cells.

You should have an understanding of the basic principles of animal cell culture

Stem Cell Training in a State-of-the-Art Laboratory

Mitomycin C treatment of Mouse Embryonic Fibroblasts (MEFs)

Clonal Nature of Spontaneously Immortalized 3T3 Cells

Feeder Cell Preparation Protocols Protocol Links

This standard operating procedure (SOP) was adapated from the protocols developed by the ES Cell Core Facility at the Samuel Lunenfeld Research Institute (SLRI), Mount Sinai Hospital, Toronto, Canada. This SOP describes the protocol for used to make primary mouse embryonic fibroblasts (MEFs) used as feeders for embryonic stem (mES) cell culture at the CMMR. The CMMR obtains its MEFs from the ES Cell Core Facility at SLRI.

To maintain pluripotency, mES cells are cultured on feeder cells; either MEFs, STO fibroblast cell line; or for feeder-free mES clones on gelatinized plates in the presence of leukaemia inhibitory factor (LIF). Growth media and culture conditions should be used as suggested for each ES cell line. Mitotically inactivated MEFs are used as feeders only for a long-term culture of R1 ES cells [1], typically before and after cryopreservation. Otherwise, culture of R1 ES cells for electroporation and during the selection is done on gelatinized plates. MEF cells can be made from any strain of mice including transgenic mice that express bacterial neomycin or hygromycin genes depending on the choice of selectable markers used for altering the ES cell genome. NeoR and HygroR (JR2356, JR2354) as well as the DR-4 strain (Jackson Laboratories #003208) bearing neo, puro, hyg resistance
genes and a deletion of the Hprt gene are available from Jackson Laboratories. Detailed protocols for preparation of MEF stocks and mitomycin C treated feeders for ES cell culture are presented in many publications (e.g. [2]). Our brief protocols

Source:

To maintain pluripotency, mES cells are cultured on feeder cells; either MEFs, STO fibroblast cell line; or for feeder-free mES clones on gelatinized plates in the presence of leukaemia inhibitory factor (LIF)

Stem Cell Protocols

MEF (Mouse embryonic fibroblast) Nucleofector Kits

If you have mouse ES cell experience, but no hES cell experience, this course will offer you a lot of new information and technical training vital to the successful propagation of your hES cells

Stem Cell Training Courses

Human Embryonic Stem Cell Culture Training Course

The tuition for the course has been set at $2,000

Workshop and Stem Cell Short Course Registration

In addition to the annual Hilton Head Workshop, a Stem Cell Short Course will be offered just prior to the start of the program. Registration is separate

Welcome to the 13th Annual
Hilton Head Workshop

The Human Embryonic Stem Cell Workshop (HEST) at Virginia Commonwealth University

EMBO Practical Course on Differential Proteomics
From 2-D Gel Electrophoresis to Mass Spectrometry

EMBL and Practical Courses (Europe)

Theoretical introduction into miRNA profiling

Learn the state of the art techniques used in modern developmental neuroscience

ChIP-Seq applied to the study of mammalian transcription factor binding sites and epigenetics

Protein Microarray

GenomeDx: High-density, Genome-wide Oligonucleotide Microarray Analysis

Pharmacology and Toxicology, Toronto

British scientists to test use of stem cells to treat stroke patients

Stem cells are to be injected into patients to try to repair brain damage in a medical first that will put British scientists at the forefront of research into incurable diseases

According to John M. Greally, Associate Professor, Departments of Medicine and Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY, the term epigenomics can be used to describe two areas of research: (i) the study of epigenetic regulation of gene expression using high-throughput techniques or (ii)
the study of the infl uence of DNA sequence on epigenetic regulation.
Human diseases in which epigenetic mechanisms are thought to play a part
include aging, cancer, obesity, type 2 diabetes, and mental disorders.

Source:

The Krembil Family Epigenetics Laboratory T

Epigenetics may indeed hold answers to many mysteries that classical genetic approaches have been unable to solve, said Dr. Arturas Petronis, an associate professor of psychiatry at the Center for Addiction and Mental Health at the University of Toronto.
For example, why does one identical twin develop schizophrenia and not the other? Why do certain disease genes seem to affect or "penetrate" some people more than others? Why do complex diseases like autism turn up in more boys than girls?

Celera Assembler: Adapting for the Future

Microsoft PowerPoint - Next Generation Sequencing and ChIP-seq.ppt -

Une puce à ADN (encore appelée biochip, DNA-microarray ou microarray) est un ensemble de molécules d'ADN fixées sur une surface

PRÉSENTATION GÉNOMIQUE INSTRUMENTS ET SERVICES

Les expériences d’immunoprécipitation
de la chromatine. Courtesy of Benoit Leblanc (usherbrooke.ca)

Source:

Interactions ADN-protéines

ChIP-sequencing (ChIPSeq) – a combination of chromatin immunoprecipitation and next-generation, or parallel, sequencing

Advances in Next Gen technologies have led to a number of significant papers in recent months

ChIP-Seq Profiling of Estrogen Receptor Alpha Binding Sites Using ...

Chromosomal DNA is frequently methylated or interacts with various proteins as a means of modifying gene expression

ChIPSeq and Epigenetics

Indeed, that's one of the major issues with ChIP-Seq: we need to do more controls to better understand the underlying processes which give us noise in the data

Though Sequencers Improve Performance, $10,000 Genome Remains Elusive in '08, Survey Finds

ChIP-Seq Data Analysis

Dr. M. Bornens

Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.

A basic question in studies of the genesis of cell polarity is whether the polarity is an intrinsic and permanent property of cells or
whether it is externally imposed by signals at the cell periphery

Dr. Andreas Wodarz
Dept. of Stem Cell Biology
GZMB
Justus-von-Liebig-Weg 11
37077 Göttingen
Germany

One important aspect of asymmetric cell division is the establishment of an intrinsic polarity which is the prerequisite for the asymmetric localization of proteins and mRNAs that serve as cell fate determinants. Our model system for the asymmetric division of stem cells is the embryonic neuroblast of Drosophila. Here we study the function of genes that control cell polarity, asymmetric localization of cell fate determinants and orientation of the mitotic spindle. The knowledge obtained in the Drosophila system has stimulated intense research on the participation of the orthologous genes and proteins in the asymmetric division of vertebrate stem cells.

The following videos are (left to right):
1)SNP Chip for the Sheep Genome
2)Stammzellen (from SynergyMarketing)

A collaborative team of scientists from Durham University and the North East England Stem Cell Institute (NESCI) has developed two synthetic molecules which can be used to coax stem cells to ‘differentiate’ - that is, transform into other forms of tissue. Their use could also help reduce the number of animals used in laboratory research.

Dr Stefan Przyborski, Durham University
Courtesy of gizmag.com

Methods of use - adult stem cells

Dr Stefan Przyborski, Durham University

Dr Stefan Przyborski and their research groups at Durham University, who are all members of the North East England Stem Cell Institute (NESCI)

Progress in Stem Cell Biology - In Vitro Applications for Research and Development

Meetings/Workshops on Molecular Biology in the United Kingdom

One of the challenges facing stem cell scientists is to find out how these may be re-programmed to become different tissue types

Professor Shinya Yamanaka of the Institute for Frontier Medical...

Single Factor Converts Adult Stem Cells into Embryonic-like Stem Cells

SOX2

Rückschlag für Stammzellbehandlung

Das Wissenmagazine

Die Wissenschaftler um Kaji verwendeten eine abgewandelte Genfähre, die ursprünglich aus Bakterien stammt und Plasmid genannt wird

Sichere Stammzellen: Ohne Viren zur Wunderwaffe

Um die so gennaten induzierten pluripotenten Stammzellen (kurz:iPS) zu erzeugen, hatte das team um Shinya Yamanka weder Eizellen noch Embryonen benoetigt. Um sie in aehnliche Alleskoenner zu verwandeln, wie es die embryonalen Stammzellen sind, genuegte es, vier Gene in das Erbgut der Hautzellen einzuschleusen: Oct4, Sox2, C-Myc and Klf4. Der Haken daran war nur: Viele der Tiere, denen man iPS-Zellen implantiert hatte, erkrankten wenige Wochen spaeter an Krebs.

Zwei Faktor-induzierte pluripotente Stammzellen (2F iPS)

Anfangs mussten Forscher zur Gewinnung von ipS-Zellen noch vier Gene mittels viraler Genfähren in die Körperzellen einschleusen

Wissenschaftler haben Körperzellen erstmals direkt in embryonale Stammzellen verwandelt

Hans Schöler, Direktor am Max-Planck-Institut für molekulare Biomedizin in Münster:Zwei plus eins" lautet ihre aktuelle Formel

Die kanadischen Forscher schleusten ebenfalls alle vier Gene zusammen mit dem Transposon in Bindegewebszellen des Menschen und der Maus ein. Ihnen gelang es zudem, die Genfähre zusammen mit den eingeschleusten Genen komplett aus dem Erbgut der induzierten Stammzellen wieder herauszuschneiden. Bei der Methode von Kaji ohne das Transposon verbleiben nach dem Herausschneiden noch Reste der Genfähre im Erbgut.

Nach der Umwandlung schnitten die Wissenschaftler die vier Gene wieder aus dem Erbgut der Zelle heraus

Körperzellen lassen sich mit weniger Eingriffen in eine Art embryonalen Zustand zurückversetzen als bisher angenommen

Nun haben deutsche Wissenschaftler das Rezept zur Gewinnung dieser iPS-Zellen (induced Pluripotent Stem Cells, übersetzt etwa künstlich erzeugte pluripotente Stammzellen)

Ein einfacherer Weg zu Alleskönner-Zellen

Der Gebrauch von Retroviren ist problematisch, da diese die Eigenschaft besitzen, ihr eigenes Erbgut irgendwo in das Genom der zu verjüngenden Zelle einzubauen

Seit 2004 ist Hans Schöler Direktor am Max-Planck-Institut für molekulare Biomedizin in Münster. Er gilt als einer der renommiertesten deutschen Stammzellforscher

‘piggyBAC’ and Induzierte pluripotente Stammzellen weiter entschärft!

US-Forscher machen aus Hautzellen Stammzellen - ohne gefährliche Viren

Meldung des Tages: Stammzellen ohne Viren

Der Cocktail aus Oct4, Klf4, Sox2 und c-Myk noch ...

Stammzellforschung und Zelltherapie

Oct-4 is an abbreviation of Octamer-4

Transcriptional Regulation of Nanog by OCT4 and SOX2

The Sleeping Beauty Transposon™ System (SBTS) is a non-viral carrier of genetic information

Gene Delivery Technology —

The Sleeping Beauty Transposon™ System (SBTS) is a non-viral carrier of genetic information that can insert a gene into vertebrate (human) chromosomes in order to confer a new function or replace a defective gene. Four patent applications regarding the use of this transposon for introducing new DNA into the chromosomes of a cell have been filed, with the base patent* issued in December 2002.
* United States Patent 6,489,458; Hackett , et al. December 3, 2002; DNA-based transposon system for the introduction of nucleic acid into DNA of a cell.

A Novel Gene Transfer System :

The SBTS (SB Therapeutics) shows promise as a long-lasting, safe way to insert genes into the chromosomes of cells without using a viral vector.

The Sleeping Beauty Transposon System is a dynamic biological device
consisting of:
• The SB Transposon.
• The SB Transposase – specific to the SB Transposon.
• A therapeutic gene.

The Sleeping Beauty transposon carrying the therapeutic gene is "cut" out of the plasmid vector by the engine of this machine (the transposase) and then "pasted" directly into the chromosome. The operation of this biological nano machine is unique and proprietary to DGI.

Source:

Discovery Genomics, Inc. (DGI)

Sleeping Beauty 'Jumping Gene' Shows Promise For Sickle Cell Gene Therapy

Sleeping Beauty Transposon-Mediated Gene Therapy for Prolonged ...

Sleeping Beauty (SB) is a transposon system genetically reconstructed from teleost fish that mediates chromosomal integration of DNA sequences by a cut-and-paste mechanism

Sleeping Beauty -based gene therapy with indoleamine 2,3 ...

Applications of Sleeping Beauty transposons for nonviral gene therapy

The Sleeping Beauty (SB) transposon system represents a new vector for non-viral gene transfer that melds advantages of viruses and other forms of naked DNA transfer

Postintegrative Gene Silencing within the Sleeping Beauty ...

Sleeping Beauty-Mediated Suicide Gene Therapy of Hepatocellular Carcinoma

Scientists wake up ancient Sleeping Beauty jumping gene

Molecular Invasion - Sleeping Beauty Affects Cell Cycle of Host Cell

Lung-directed gene therapy in mice using the nonviral Sleeping Beauty transposon system

Name of Videos from left to right:
1)Jerome Zack: Creating iPS Cells
2)Mark Mercola: Differentiating embryonic stem cells into adult tissues

Stochasticity and the Molecular Mechanisms of Induced Pluripotency

Stochasticity and the Molecular Mechanisms of Induced Pluripotency

Ben D. MacArthur, Colin P. Please, and Richard O. C. Oreffo1

The generation of induced pluripotent stem cells from adult somatic cells by ectopic expression of key transcription factors holds significant medical promise.
However, current techniques for inducing pluripotency rely on viral infection and are therefore not, at present, viable within a clinical setting. Thus, there is now a need to better understand the molecular basis of stem cell pluripotency and lineage specification in order to investigate alternative methods to induce pluripotency for clinical application. However, the complexity of the underlying molecular circuitry makes this a conceptually difficult task. In order to address these issues, we considered a computational model of transcriptional control of cell fate specification. The model comprises two mutually interacting sub-circuits: a central pluripotency circuit consisting of interactions between stem-cell specific transcription factors OCT4, SOX2 and NANOG coupled to a differentiation circuit consisting of interactions between lineage-specifying master genes.

...The flexibility in the differentiation hierarchy is commonly known as lineage plasticity [3] and is perhaps most dramatically demonstrated by molecular reprogramming of adult somatic cells into so-called induced pluripotent stem (iPS) cells, which express the genetic and phenotypic characteristics of pluripotent ES cells. Since iPS cells potentially provide a patient-specific source of pluripotent stem cells they possess significant clinical potential.Furthermore, since they are derived from adult somatic cells which are easily harvested through biopsy, the generation and clinical use of iPS cells is not associated with the same ethical controversies as human ES cells, although they are associated with significant alternative ethical issues.

Source:

Stochasticity and the Molecular Mechanisms of Induced Pluripotency

Pluripotency can be defined as the ability to contribute cellular material to all cell lineages present in the adult

Induced Pluripotent Stem Cells Generated from Reprogramming Differentiated Cells by Defined Factors

Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection

Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors>

Pluripotency Redux — Advances in Stem-Cell Research

MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation

Transcriptional regulation of nanog by OCT4 and SOX2

Forced expression of Sox2 or Nanog in human bone marrow derived mesenchymal stem cells

Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells

Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors

The stepwise specification of embryonic stem cells to hematopoietic fate is driven by sequential exposure to Bmp4, activin A, bFGF and VEGF

Networking of WNT, FGF, Notch, BMP, and Hedgehog signaling pathways during carcinogenesis

Differentiation of Human Embryonic Stem Cells in Serum Free Medium Reveals Distinct Roles for BMP4, VEGF, SCF and FGF2 in Hematopoiesis

Enhanced efficiency of generating induced pluripotent stem (iPS ...

Noboru Sato:Assistant Professor of Biochemistry Welcome to the Stem Cell World!

Noboru Sato: Different Levels of Stem Cell State

The significance of induced pluripotent stem cells for basic research and clinical therapy

Concise Review: Epigenetic Mechanisms Contribute to Pluripotency and Cell Lineage Determination of Embryonic Stem Cells

Recent advances in cardiovascular regenerative medicine: the induced pluripotent stem cell era

Pluripotency, the ability of a cell to give rise to all of the cells of an organism, is a fascinating and mysterious characteristic of embryonic stem (ES) cells

Pluripotency Redux — Advances in Stem-Cell Research

Shanghai Team Analyzes Mechanism that Enables Adult Cells to Behave Like Embryonic Stem Cells

Deconstructing Pluripotency

POU5F1 (OCT3/4) Identifies Cells with Pluripotent Potential in Human Germ Cell Tumors

Pioneering Stem Cell Biologist Dr. Rudolf Jaenisch, M.D., Joins Founding Scientific Team of Fate Therapeutics

Professor Rudolf Jaenisch. Courtesy of scienceprogress.org

LA JOLLA, Calif. & CAMBRIDGE, Mass.--(BUSINESS WIRE)--Fate Therapeutics, Inc. announced today that Dr. Rudolf Jaenisch, M.D., founding member of the Whitehead Institute for Biomedical Research and professor in the Department of Biology, Massachusetts Institute of Technology, has joined the Company’s internationally recognized team of scientific founders dedicated to understanding stem cell biology in human physiology and disease. Dr. Jaenisch is credited with being one of the first to discover the revolutionary mechanisms for “reprogramming” fully-mature adult cells to a stem-like state. The creation of these “reprogrammed” cells, known as induced pluripotent stem (iPS) cells, provides numerous advantages over stem cells sourced from human embryos and has ushered in a new paradigm in stem cell research for modeling human diseases, discovering and testing conventional pharmaceuticals and developing personalized cell replacement therapies.

Source:

Pioneering Stem Cell Biologist Dr. Rudolf Jaenisch, M.D., Joins Founding Scientific Team of Fate Therapeutics

Professor Rudolf Jaenisch. Courtesy of mdc-berlin.de

Links:

Breakthrough produces Parkinson's patient-specific stem cells free of harmful reprogramming genes

Reprogrammed cells reduce Parkinson's symptoms in rats

Breakthrough method produces Parkinson’s disease patient-specific stem cells free of harmful reprogramming genes

Fate Therapeutics Bolsters Academic Stem-Cell IP Portfolio with Whitehead Institute License

Die Gruppe um den deutschstämmigen Genpionier Rudolf Jaenisch vom Whitehead-Institut am Massachusetts Institute of Technology, die mit einem genchirurgischen Verfahren aus Hautzellen von fünf Parkinson-Patienten sogenannte induzierte Stammzellen (iPS) gewonnen hat

Prof. Rudolf Jaenisch mit Max-Delbrück-Medaille geehrt

IRVINE--- World Renowned Stem Cell Researcher Dr. Rudolf Jaenisch Joins PrimeGen Biotech Scientific Advisory Board

Professor Jaenisch: "The goal is to do without human oocytes in the future."

Pioneering Stem Cell Biologist Dr. Rudolf Jaenisch, M.D., Joins Founding Scientific Team of Fate Therapeutics

Single virus used to convert adult cells to embryonic stem cell-like cells

World Renowned Stem Cell Researcher Dr. Rudolf Jaenisch Joins PrimeGen Biotech Scientific Advisory Board

Nuclear Cloning and Cell Therapy: Fact and Fiction

Clonation for disorders

Rudolf Jaenisch:"Genes have always received far more than the lion’s share of credit for determining our physical characteristics—genotype determines phenotype, as the old biological cliché has it. But genes aren’t everything."

But genes aren’t everything. Brain, liver, heart, skin, and nerve cells, just to name a few, all have the same set of genes—the same DNA in the chromosomes—and yet entirely different structures and functions. So why does the DNA of one cell direct it to become a neuron, while the DNA of another might preordain a skin cell, or a liver cell, or any other cell type in the human body? This is the world of " epigenetic" gene regulation—the molecular signals outside the genome that determine which genes will be activated and which silenced during development and cell proliferation.

Source:

Rudolf Jaenisch, Science Watch Newsletter Interview

Virus-free Embryonic-like Stem Cells Made From Skin Of Parkinson's Disease Patients

piggyBac transposition reprograms induced pluripotent stem cells in a safer way

Stem Cell Breakthrough May Eliminate Need for Embryonic Cells

Stem cells 'from adult cells'

Researchers piggyback to safer reprogrammed stem cells

سلول های بنيادی تحريک شده (IPS)

پرتوان القايي

سلول‌هاي بنيادي پرتوان القايي
SabzTec

Working to generate the so-called induced pluripotent stem cell or iPS cells to match a variety of diseases

Induced pluripotent stem cell

Skin Cell Into Sperm Or Egg? Human Induced Plurtipotent Stem Cells Reprogrammed Into Germ Cell Precursors